Cell Cycle
It is now possible to measure luminescence of single PER-LUC labeled cells using cooled CCD cameras. With their high quantum efficiency and low noise, the cameras can extract significant measurements of luminance from the handful of numbers of photons emitted by single cells. Analyzing the image series from such experiments can laborious, however. Each of the many cells must be identified and then tracked through the image series, a difficult prospect given that the cells can move from image to image, and — during the dim phase of the circadian day — can completely disappear from view. Cell Cycle facilitates the image analysis. Movie Playback. Cell Cycle assembles the image stack into a movie that can be stepped through or played back and forth at different speeds using standard video controls. Watching the movie makes is easy to pick out the cells that are suitable for analysis. The program automatically adjusts the brightness of the video during playback to compensate for the gradual decline in luminescence of the cells over time.
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